Mouse polycomb targets in gonadal development

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06917
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical genome science
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Katoh-Fukui Yuko 国立研究開発法人国立成育医療研究センター, 分子内分泌研究部, (非)研究員 (50342639)
• Co-Investigator(Kenkyū-buntansha): 高田 修治 国立研究開発法人国立成育医療研究センター, システム発生・再生医学研究部, 部長 (20382856)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Polycomb / Mouse / Sex determination / Gonadal development

Outline of Final Research Achievements

In the course of embryonic development, he polycomb group (PcG) proteins are major chromatin components that are involved in the regulation of target genes. Mice lacking the function of the PcG protein Cbx2 show defects in gonadal, adrenal, and splenic development. In particular, XY Cbx2 KO mice develop ovaries but not testes, and the gonads are hypoplastic in both sexes. How Cbx2 regulates target genes in development of these tissues remains largely unknown. In this project, we tried to identify the target region of Cbx2 in embryonic gonad, and further delete the region by CRISPR-Cas9 genome editing in mice, to analyze the molecular mechanisms of Cbx2 in developing gonad.

Free Research Field

ゲノム生物学