2017 Fiscal Year Final Research Report
Epigenomic landscapes governed by distinct PRC2s
| Project/Area Number |
15K06942
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University (2017)
Hokkaido University (2015-2016) |
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Principal Investigator |
Nagao Koji 大阪大学, 理学研究科, 准教授 (60426575)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | エピジェネティクス |
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| Outline of Final Research Achievements |
Polycomb repressive complex 2 (PRC2) play a key role in silencing developmentally regulated genes to establish and maintain facultative heterochromatin. The core PRC2 complex comprises four components (EZH1/2, SUZ12, EED and RBBP4/7) and methylates histone H3 at Lys 27 through its enzymatic subunit EZH1/2. In addition, five regulatory components have been identified in mammalian PRC2. However, how mammalian PRC2 regulates various genomic sites and collaborates with other chromatin modulators remains unclear. Reconstitution of PRC2 in Sf9 insect cells using intact or truncated components resulted in subunit organization of PRC2.
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| Free Research Field |
分子生物学
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