2017 Fiscal Year Final Research Report
The Regulation of Replisome Dynamics during Replication Fork Collapse
| Project/Area Number |
15K06955
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | DNA複製フォーク / レプリソーム / 無細胞複製系 / CDK / ユビキチン化 / Mre11ヌクレアーゼ / 細胞周期 |
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| Outline of Final Research Achievements |
The DNA Replication machinery, or replisome is displaced from chromatin at the end of DNA replication and during the collapse of stalled replication forks. In this study, I aimed to elucidate the molecular mechanism of the replisome displacement during fork collapse by utilizing cell-free DNA replication system, and found that the replisome displacement was efficiently induced at stalled forks by enforcing cell cycle transit from interphase into mitotic phase. I also demonstrated that this mitotic displacement pathway is a novel one that requires CDK activity, ubiquitylation and Mre11 nuclease activatey.
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| Free Research Field |
分子生物学、生化学
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