2016 Fiscal Year Research-status Report
Thermodynamic basis of life: protein-carbohydrate interaction
| Project/Area Number |
15K06962
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| Research Institution | Kyushu University |
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Principal Investigator |
CAAVEIRO Jose 九州大学, 薬学研究院, 准教授 (00536732)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Glycan / Sugar / Antobody / Enzyme / Pore forming protein / X-ray crystallography / Thernodynamics / Kinetics |
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| Outline of Annual Research Achievements |
1. Research activities were advanced in different fronts with the goal of maximum productivity. Because I started a new laboratory at the University of Kyushu, in which I am the Principal Investigator, the research was concentrated in the publication of results. The experimentation had to to be slowed down momentarily and concentrated to essential aspects of the research. In particular, I have concentrated my efforts towards the determination of important crystal structures. In the fiscal year 2016/17 expenditures were reduced because of my transfer to Kyushu University. The money saved in 2016/17 will be used in fiscal year 17/18 to acquire essential scientific material to complete the project. I think this strategy is working really well, and has led to better than expected scientific output.
2. During the fiscal year 2016/17 I had the opportunity to present our research at various locations oversees. Importantly, the presentations were done by invitation. 3. I was also invited to Hokkaido University where I presented an important part of the results obtained in this project.
4. During the fiscal year 2016/17 the data of my research was published in various prestigious international journals, as detailed in the corresponding section.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
Three invited talks (London by the Royal Society, and Chile), and seven papers is a extraordinary achievement for this project in my opinion.
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| Strategy for Future Research Activity |
To obtain additional structural and thermodynamic data.
Publish 3-5 more papers, including one review.
Attend meetings in Japan and abroad.
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| Causes of Carryover |
In 2016/17 I moved from The University of Tokyo to Kyushu University to start an independent research laboratory, in which I am the Principal Investigator. I have saved some money to be used on 2017/18 more efficiently in my new laboratory. This strategy has not slowed down the project in 2016/17.
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| Expenditure Plan for Carryover Budget |
The additional money will be used, entirely, for the purchase of goods and articles. This budget is essential for the completion of this project.
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[Journal Article] Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin2017
Author(s)
Kudo, S., Caaveiro, J.M.M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., and Tsumoto, K.
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Journal Title
Sci. Rep.
Volume: 7
Pages: 39518
DOI
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Peripheral membrane interactions boost the engagement by an anti HIV-1 broadly neutralizing antibody2017
Author(s)
Rujas, E., Caaveiro, J.M.M., Insausti, S., Garcia-Porras, M., Tsumoto, K., and Nieva, J.L.
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Journal Title
J. Biol. Chem.
Volume: 292
Pages: 5571-5583
DOI
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Functional contacts between MPER and the anti-HIV-1 broadly neutralizing antibody 4E10 extend into the core of the membrane2017
Author(s)
Rujas, E., Insausti, S., Garcia-Porras, M., Sanchez-Eugenia, R., Tsumoto, K., Nieva, J.L., and Caaveiro, J.M.M.
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Journal Title
J. Mol. Biol.
Volume: 429
Pages: 1213-1226
DOI
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Identification of a membrane-bound prepore species clarifies the lytic mechanism of actinoporins2016
Author(s)
Morante, K., Bellomio, A., Gil-Carton, D., Redondo-Morata, L., Sot, J., Scheuring, S., Valle, M., Gonzalez-Manas, J.M., Tsumoto, K., and Caaveiro, J.M.M.
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Journal Title
J. Biol. Chem.
Volume: 291
Pages: 19210-19219
DOI
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Differential effect of membrane composition on the pore forming ability of four different sea anemone actinoporins2016
Author(s)
Garcia-Linares, S., Rivera-de-Torre, E., Morante, K., Tsumoto, K., Caaveiro, J.M.M., Gavilanes, J.G., Slotte, J.P., and Martinez-del-Pozo, A.
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Journal Title
Biochemistry
Volume: 55
Pages: 6630-6641
DOI
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface2016
Author(s)
Rujas, E., Caaveiro, J.M.M., Partida-Hanon, A., Gulzar, N., Morante, K., Apellaniz, B., Garcia-Porras, M., Bruix, M., Tsumoto, K., Scott, J.K., Jimenez, M.A., and Nieva, J.L.
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Journal Title
Sci Rep.
Volume: 6
Pages: 38177
DOI
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Pore formation assisted by lipids2016
Author(s)
Caaveiro, J.M.M.
Organizer
Membrane pores: from structure and assembly, to medicine and technology, The Royal Society
Place of Presentation
London, UK
Year and Date
2016-06-27 – 2016-06-27
Int'l Joint Research / Invited
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