2017 Fiscal Year Final Research Report
The structure analysis of PBCV-1 by the combination of X-ray crystallography and coherent X-ray diffraction imaging
| Project/Area Number |
15K06971
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Ｘ線結晶解析 / コヒーレントＸ線イメージング |
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| Outline of Final Research Achievements |
Studies aimed at the structure analysis of a giant virus, PBCV-1 by the combination of X-ray crystallography and coherent X-ray diffraction imaging was carried out. The single crystals of PBCV1 have not been obtained yet, but the technical improvements were proceeded using the other virus crystals as a model. The high-resolution diffraction patterns were successfully observed from viral fragile crystals.
In the experiment of coherent X-ray diffraction imaging, so many diffraction patterns from PBCV-1 were collected and processed for 2D imaging. The data selection of diffraction data from huge data set is carrying out aimed to the 3D reconstruction of PBCV-1.
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| Free Research Field |
構造生物学
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