The molecular mechanisms of interactions between immune cells and its target antigens involved in the vertebrate tissue remodeling

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06992
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Niigata University
• Principal Investigator: Izutsu Yumi 新潟大学, 自然科学系, 教授 (20301921)
• Co-Investigator(Kenkyū-buntansha): 伊藤 道彦 北里大学, 理学部, 准教授 (90240994)
• Research Collaborator: 小林 遙香 , 大学院生 ; 浅野 亮人 , 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: タンパク質 / 発現調節 / 発生・分化 / 組織・細胞 / 両生類 / アポトーシス / T細胞 / 免疫学

Outline of Final Research Achievements

We have previously reported that Ouro proteins and adult T cells are involved in the process of the tail degeneration during metamorphosis (Mukaigasa et al….. & Izutsu, 2009). We presented here the loss-of-function and rescue analyses to investigate whether or not the immune T cells are necessary for the tadpole’s tail regression via Ouro proteins during metamorphosis. To remove the T cells from the tadpoles, we used a monoclonal antibody against Xenopus T cell named XT-1. After XT-1 treatment with pre-metamorphic tadpoles, degeneration of Ouro-expressing tail tissues was significantly inhibited. To investigate T cell potency upon degeneration of the tadpole tail tissues, the rescue experiment was performed by transfer of T cells obtained from the J strain frogs into tadpoles, in which the T cells were depleted. It was succeeded in providing degenerated tails of a transgenic.

Free Research Field

発生生物学、免疫学