2017 Fiscal Year Final Research Report
Substrate recognition mechanism of the endoplasmic reticulum-localized mannosidase EDEM
| Project/Area Number |
15K06996
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Okada Tetsuya 京都大学, 理学研究科, 助教 (70378529)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | タンパク質分解 / マンノシダーゼ |
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| Outline of Final Research Achievements |
Mannose trimming in the endoplasmic reticulum required for glycoprotein degradation is carried out by the EDEM family proteins (EDEM 1/2/3). In this study, we clarified the functional significance of EDEM2 in the mannose trimming reaction and identified three cysteine residues essential for the mannosidase activity of EDEM2. We also identified an candidate for the partner protein of EDEM2. In addition, we found that severely misfolded glycoproteins are forcibly degraded even in the absence of EDEM-mediated mannose trimming.
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| Free Research Field |
細胞生物学
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