Comprehensive investigation of molecular mechansim of intracellular localization of DG kinase as an example of general enzmes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kobe University
• Principal Investigator: Shirai Yasuhito 神戸大学, 農学研究科, 教授 (60263399)
• Co-Investigator(Renkei-kenkyūsha): TSURUTA HIROKI 神戸大学, 連携創造本部, 准教授 (20346282)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脂質キナーゼ / ジアシルグリセロール / 可視化 / 結合タンパク質 / 細胞内局在変化 / チロシンリン酸化 / フォスファチジン酸

Outline of Final Research Achievements

In this study, molecular mechanism of nuclear-cytoplasm shuttling of diacylglycerol kinase alpha (DGKa) was investigated. We found that RVH-EF region directly bound to C1 domain which functions as nuclear localization signal (NLS) nteraction was enhanced by tyrosin phosphorylation at Y218, resulting weaken the function of C1 domain as NLS. We also identified cyclin dependent kinase 12 and Rheb as binding proteins of DGKa in the nucleus and the cytoplasm, respectively. The former seems to work to stop the cell cycle and the latter seems to contribute the proliferation.

Free Research Field

生化学