The novel mechanism of regulation of microtubule dynamics by dynamin

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Hiroshima University
• Principal Investigator: Hamao Kozue 広島大学, 理学研究科, 准教授 (10403578)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ダイナミン / 微小管 / ダイナミクス

Outline of Final Research Achievements

Dynamin has been firstly identified as a microtubule-associated protein. However, the function of dynamin bound to microtubules is still unknown. This study aimed to elucidate the molecular mechanism of microtubule regulation by dynamin. It was revealed that depletion of dynamin stabilizes microtubules and the stabilized microtubules in dynamin-depleted cells are decreased by overexpression of GFP-dynamin-wild type or proline rich-deleted dynamin. On the other hand, overexpression of each GFP-dynamin mutant without GTPase, middle, plekstrin homology or GTPase effector did not decrease the stabilized microtubules. In addition, it was showed that overexpression of dynamin mutant capable of stabilizing microtubules increases the staining of EB1, one of microtubule plus-end tracking proteins, without changing the CLIP-170 or CLASP2. This study suggests that dynamin could regulate microtubules through its own GTPase activity and EB1.

Free Research Field

細胞生物学，生化学