Analysis of effects of lipids on function and structure of membrane proteins

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Ehime University
• Principal Investigator: Nozawa Akira 愛媛大学, プロテオサイエンスセンター, 講師 (30432800)
• Co-Investigator(Renkei-kenkyūsha): TOZAWA Yuzuru 埼玉大学, 大学院・理工学研究科, 教授 (90363267)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ミトコンドリアキャリア / カルジオリピン / プロテオリポソーム

Outline of Final Research Achievements

In this study, by using mitochondrial carriers and a lipid, cardiolipine, which is known to interact with the mitochondrial carrier, as a model membrane protein-lipid system, we clarified roles of lipids against the function and structure of the membrane protein. In this study, the effect of cardiolipin on transport activity of yeast and malaria parasite mitochondrial carriers was analyzed. As a result, it was clarified that addition of cardiolipin increases the transport activity of the mitochondrial carriers localized in mitochondrial membrane, but such a effect was not observed in the mitochondrial carriers present in other membrane systems.

Free Research Field

膜輸送体タンパク質の機能解析