Function of the AAA ATPase Cdc48 in mitochondrial morphology regulation

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kumamoto University
• Principal Investigator: Esaki Masatoshi 熊本大学, 発生医学研究所, 助教 (70437911)
• Research Collaborator: Chowdhury Abhijit 熊本大学, 発生医学研究所, 大学院生 ; Islam Tanvir Md. 熊本大学, 発生医学研究所, 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ミトコンドリア / 膜融合 / タンパク質分解 / AAA ATPase / 分子シャペロン

Outline of Final Research Achievements

Mitochondria form tubular structures during vegetative growth and undergo consecutive division and fusion. We have shown that the AAA ATPase Cdc48 is required for the fusion and for turnover of the fusion-responsible mitochondrial outer membrane protein Fzo1. In this study, we found that two Cdc48-interacting proteins, Ubp3 and Ubx2, are involved in mitochondrial fusion and Fzo1 turnover, respectively. Ubp3 facilitates degradation of Ubp12, a down-regulation factor for the fusion-responsible modification of Fzo1, thereby facilitating mitochondrial fusion. By contrast, Ubx2 facilitates Fzo1 turnover without affecting mitochondrial morphology.

Free Research Field

分子細胞生物学