The elucidation of the mechanisms of ion selectivity and ion transport pathway of Mrp type Na+/H+ antiporter

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Toyo University
• Principal Investigator: Ito Masairo 東洋大学, 生命科学部, 教授 (80297738)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: アンチポーター / Mrp / イオン選択性 / 好アルカリ性細菌 / ｐHホメオスタシス / Bacillus / カルシウムイオン / ナトリウムイオン

Outline of Final Research Achievements

In this study, clarification of the ion transport route of Mrp type Na+/H+ antiporter was carried out. It has been reported that Ca2+ is transported in addition to Na+ and K+ among homologous proteins of this enzyme. Therefore, we attempted to elucidate the purpose of this research by using three different Mrp homologs of cations to be transported. As a result, it was revealed that the coupling cation transport route of this enzyme was formed at the fifth transmembrane segment of MrpA and the twelfth transmembrane segment of MrpD which are the interface between the two subunits.

Free Research Field

極限環境微生物学