2017 Fiscal Year Final Research Report
Nucleolar stress sensing in the nucleolus
| Project/Area Number |
15K07020
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kobe University (2016-2017)
National Institute of Infectious Diseases (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | シグナル伝達 / 核小体ストレス |
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| Outline of Final Research Achievements |
The nucleolus is a signaling center for nucleolar stress, which is induced by impaired ribosome biogenesis. PICT1 is known to play critical roles in the nucleolar stress response. In this study, I performed siRNA-mediated gene silencing, affinity pulldown, and in vitro PICT1 degradation assay to identify a stabilizing factor(s) which binds to and stabilizes PICT1 protein. I also conducted immunoprecipitation assay to analyze nucleolar stress-induced dissociation of PICT1 and candidate stabilizing factor(s). As the result, I successful found that RPX directly bound to PICT1 and repressed PICT1 degradation by 20S proteasome in vitro. In addition, immunoprecipitation assay showed that the nucleolar stress significantly decreased PICT1-RPX interaction, suggesting RPX is an upstream factor which regulates PICT1 stability and the nucleolar stress response.
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| Free Research Field |
生化学・分子細胞生物学
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