Role of polymeric OPN on fibrosis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Hiroshima University
• Principal Investigator: Nishimichi Norihisa 広島大学, 保健管理センター, 研究員 (00583486)
• Co-Investigator(Kenkyū-buntansha): 仙谷 和弘 広島大学, 医歯薬保健学研究科(医), 講師 (30508164) ; 横崎 恭之 広島大学, 保健管理センター, 准教授 (80210607) ; 外丸 祐介 広島大学, 自然科学研究支援開発センター, 教授 (90309352) ; 佐久間 哲史 広島大学, 理学研究科, 特任講師 (90711143)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: オステオポンチン / 線維化 / 翻訳後修飾 / ゲノム編集 / 抗体

Outline of Final Research Achievements

In this study, we examined to reveal the role of post-translational modification such as thrombin-cleaved and transglutaminase-polymerized osteopontin on fibrosis. As a result, at first, we succeeded generation of mice expressing thrombin-cleaved formed or transglutaminase-polymerized formed of osteopontin by genome editing. But the importance of these post-translational modification of osteopontin on fibrosis was not cleared in this study. We were successful to construct polymeric human osteopontin specific ELISA system.

Free Research Field

免疫学、分子生物学、細胞接着分子、細胞外マトリックス