Analysis of molecular mechanismthat regulate extracellular localization of basement membranes .

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Ariake National College of Technology (2017); National Institute of Genetics (2015-2016)
• Principal Investigator: Ihara shinji 有明工業高等専門学校, 創造工学科, 准教授 (70373272)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 基底膜 / 小胞体 / GPI / フォールディング / 線虫

Outline of Final Research Achievements

The basement membranes (BM) are dense sheets structure of protein complex composed by secreted proteins, providing structural support, barrier function, and maintenance of cell polarity. Caenorhabditis elegans (C. elegans) is a semi-transparent soil nematode that is useful for fluorescence tag to visualize proteins. To know different localization patterning of BM structural proteins in vivo, we visualized type IV Collagen that is major basement membrane protein. Using these transgenic lines, we have initiated ethyl methanesulfonate mutagenesis screening to isolate mutants showing aberrant accumulation or distribution of BM components. Cloning of the relevant gene revealed that it encodes pigN that is generally required for glycosylphosphatidylinositol (GPI) maturation. Interestingly, protein accumulations have not observed in the mutants of other PIG genes, indicating that PIGN have unique function for proteins secretion.

Free Research Field

細胞生物学