The role of the nucleocytoplasmic transport during embryogenesis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07069
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Nihon University (2016-2017); National Institutes of Biomedical Innovation, Health and Nutrition (2015)
• Principal Investigator: YASUHARA Noriko (垣内徳子) 日本大学, 文理学部, 准教授 (90423152)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 核輸送

Outline of Final Research Achievements

Although the nucleocytoplasmic transport is supposed to be involved in mammalian embryogenesis, not all of the molecular mechanism has been elucidated. We tried to investigate the role of the nucleocytoplasmic transport in mouse embryogenesis via phenotype analysis of transgenic mice. As a result, we found that the transgenic mice exhibited some stage specific phenotype during development. We also tried to characterize the transgenic cell lines of nucleocytoplasmic transport receptors, and revealed that the receptors are involved in proliferation. Additionally, we obtained aptamers that bind specifically to nucleocytoplasmic transport receptor, by which we are planning to use as specific inhibitor when monitoring the activity of the receptors during embryogenesis.

Free Research Field

細胞生物学