Elucidation of the mechanism of environmental stress-specific translational regulation in plant

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07107
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: kato ko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80283935)
• Research Collaborator: YAMASAKI Shotaro
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 翻訳制御 / 環境ストレス / 植物培養細胞 / ゲノムワイド解析 / 5’UTR

Outline of Final Research Achievements

When plants are exposed to environmental stress (heat, salt etc.) the translation state changes dramatically. Translation state changes due to heat and salt stress are very similar when compared on the genome wide scale, but there are also mRNAs that differ in translation state change between two stresses such as being maintained by heat but suppressed by salt.
In this study, transient expression experiments and analysis using a transformant in which the 5'UTR of mRNA showing specific behavior was fused to a reporter gene were performed. As a result, the possibility that the CU repeat region in the 5'UTR contribute to the stress state-specific translation state change (heat suppression and salt maintenance) and the change of the translational state of mRNA under heat stress by change of transcription initiation site in response to heat stress were indicated.

Free Research Field

植物へ導入した有用遺伝子の効率的発現