Functional analysis of Rif1 in regulating muticopy gene cluster and super state of pluripotent stem cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: YOSHIZAWA Naoko (須賀田直子) 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (30344071)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Rif1 / 初期胚 / 2細胞期 / 内在性レトロウィルス / エンハンサー / Zscan4 / ヒストン修飾 / DNAメチル化

Outline of Final Research Achievements

Mouse embryonic stem (ES) cells contain a small population expressing zygotic genes usually transcribed at 2-cell stage (2CG). Base on the previous finding, 2CG-high cells induced by Rif1 knockdown were precisely analyzed in this study. The main results were as follows; 1. mRif1 knockdown lead to massive derepression of 2CG and endoretrovirus Merv-L. 2. DNA methylation at Zscan4 loci was not substantially reduced. 3. H3K27Ac was noticeably increased in Zscan4 gene loci and formed enhancer-like structure for at least 20 kb. Futheremore, Transient expression of mRif1 deletion mutant without N-terminal HEAT repeat structure could induce 2CG-high states.

Free Research Field

分子生物学