2017 Fiscal Year Final Research Report
Functional analysis of the genes involved in starch degradation in rice leaves and stems and their varietal differences.
| Project/Area Number |
15K07276
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Crop production science
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| Research Institution | Meijo University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | イネ / 葉鞘 / デンプン分解 / α-アミラーゼ / β-アミラーゼ |
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| Outline of Final Research Achievements |
Starch accumulated in rice stems and leaves before heading is degraded at the post-heading stage and utilized for the grain filling. In the present study, the repressed expression of both OsBAM2 and OsBAM3 encoding the β-amylase isoforms inhibited the decrease in starch content in the leaf sheaths at the post-heading stage. The transient expression of chimeric protein with GFP indicated that the other β-amylase isogene, OsBAM5, encode plastid-targeted protein. In addition, the repression of OsBAM5 expression caused excess accumulation of starch in the leaf sheaths after heading. Triple knockdown lines for OsBAM2, OsBAM3 and OsBAM5 were generated in Nipponbare. Furthermore, the knockdown lines of RAmy2A, an α-amylase isogene, which is highly expressed in the leaf sheaths of high yield variety Takanari, were successfully generated in Takanari.
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| Free Research Field |
作物学
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