Analysis of pedigree record of wasabi cultivars and construction of DNA barcoding based on the comparison of whole chloroplast genome sequences

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07289
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Gifu University
• Principal Investigator: YAMANE Kyoko 岐阜大学, 応用生物科学部, 准教授 (00405359)
• Co-Investigator(Kenkyū-buntansha): 矢野 健太郎 明治大学, 農学部, 専任教授 (00446543)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: wasabi / chloroplast / genome / sequence / DNA marker / cultivar / genotyping

Outline of Final Research Achievements

Using Sanger's method, we successfully sequenced the whole chloroplast genome of 6 wasabi accessions for the first time in the world. There were only two polymorphic sites at complete chloroplast sequences of 150kb among “Fujidaruma” and “Simane-3 go”. It showed the superiority for the whole chloroplast genome analysis. The present results of comparative study for 234 accessions of wasabi in Japan showed that only three types of maternal germplasm were detected in wasabi cultivars. It does not contradict the results. In this study, we developed DNA markers for discriminating type of three major wasabi cultivars.

Free Research Field

植物遺伝育種学