Construction of a High-Density Linkage Map for loquat Using RAD-Seq and development of DNA markers on Fruit traits

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07293
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Saga University
• Principal Investigator: Shinji FUKUDA 佐賀大学, 農学部, 准教授 (70503770)
• Co-Investigator(Kenkyū-buntansha): 永野 幸生 佐賀大学, 総合分析実験センター, 准教授 (00263038)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Toshiya 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 上席研究員 (60355360)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ビワ / 連鎖地図 / QTL解析 / 育種 / DNAマーカー

Outline of Final Research Achievements

We constructed a high-density genetic linkage map of bronze loquat for a three-way cross of loquat × 78-51by using single nucleotide polymorphism markers generated by RAD-Seq, with the combination of simple sequence repeat markers, which have been used in the previous study. The map of bronze loquat consisted of 960 loci including 793 SNPs, 166 SSRs (98 apple, 8 loquat and 60 pear SSRs), and the loquat canker resistance gene Pse-a. This map covered 17 linkage groups over a total length of 1707.4 cM with an average distance of 1.78 cM between markers. The number of linkage groups corresponded to the basic chromosome number. The analysis of the markers that can be anchored to apple draft genome showed that almost all loquat linkage groups were aligned to the apple draft genome. The constructed map will enable us to determine the location of quantitative trait loci and genes of interest, and to analyze genome synteny in the tribe Pyreae, subfamily Spiraeoideae of the family Rosaceae.

Free Research Field

果樹園芸