RNA sequence analysis of flower induction mechanisms by gibberellins in Phalaenopsis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Nihon University
• Principal Investigator: KUBOTA Satoshi 日本大学, 生物資源科学部, 教授 (60328705)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ファレノプシス / ジベレリン / 花成誘導

Outline of Final Research Achievements

Gene expression analysis by RNA-Seq was conducted to investigate the flowering induction mechanism of Phalaenopsis by GA. When GA was applied at apical bud, flower-related genes such as SPL, AP2, FT, AP1 and GA biosynthesis and signal transduction genes such as GA20ox, GA3ox, GA2ox, GID1, DELLA were greatly different. The expression level of PhFT and ORAP13 at the shoot apex increased 840 fold and 8.3 fold at 7 weeks after the beginning of GA application compared with those of before GA application, respectively. Therefore, there are specific mechanism that PhFT was remarkably expressed at the shoot apex, followed by the expression level of ORAP13 was also increased during the flower induction at apical bud by GA.

Free Research Field

花き園芸学