Virome of the phytopathogenic fungi: metagenomic analysis and the possible utilization

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07312
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Okayama University
• Principal Investigator: Kodno Hideki 岡山大学, 資源植物科学研究所, 准教授 (40263628)
• Co-Investigator(Kenkyū-buntansha): 鈴木 信弘 岡山大学, 資源植物科学研究所, 教授 (70206514)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ウイルス / 菌類 / メタゲノム解析 / 次世代シークエンサー / 絶対寄生菌 / うどんこ病菌 / クローバー / トティウイルス

Outline of Final Research Achievements

The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. However, virus discovery in obligate biotrophs such as powdery mildews (ascomycetes) and rust fungi (basidiomycetes) is still very limited. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least nine novel totivirus (genus Totivirus) in this fungus. Similar totvirus-like sequences are found in public transcriptome shotgun assembly (TSA) libraries of the bean rust fungi. Our data suggests that the horizontal transmission of ancestral totiviruses might have occurred across quite different fungal phyla, between powdery mildews and rust fungi and later evolved within the new host fungus separately.

Free Research Field

植物病理