Regulation of polar localization of membrane transporter

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: The University of Tokyo
• Principal Investigator: Kasai Koji 東京大学, 大学院農学生命科学研究科(農学部), 特任准教授 (80517938)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 膜輸送体 / 極性局在 / ホウ酸トランスポーター

Outline of Final Research Achievements

Arabidopsis borate transporter BOR1 is localize to the inner plasma membrane in the root cell, and the polar localization is effective to transport boron which is the micronutrient of plant from soil to the plant above ground part effectively. We introduced the sequence domain that decided inward polar localization of BOR1 into the nutrient transporter except the BOR borare transporter. After fusing with the sequence of the BOR1-C-terminal domain in the PCR2 gene which was a heavy metal transporter, PCR2 showed inward polarity. This result shows possibility that the C-terminal domain sequence of BOR1 can be used as signal sequence for inward polar localization to various plasma membrane proteins.

Free Research Field

植物分子生物学