2017 Fiscal Year Final Research Report
Analysis of the regulatory mechanism of the denitrification genes in an aerobic anoxygenic photosynthetic bacterium
Project/Area Number |
15K07351
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | The University of Tokyo |
Principal Investigator |
Arai Hiroyuki 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (70291052)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | 脱窒 / 光合成 / 一酸化窒素 / 微生物 |
Outline of Final Research Achievements |
An aerobic anoxygenic photosynthetic bacterium Roseobacter denitrificans OCh114 requires two DNR-type transcriptional regulators, DNR1 and DNR2, for anaerobic growth by denitrification. Gene expression analyses using the mutant strains of some regulatory genes revealed that DNR1, DNR2, and the denitrifications genes constitute a cascade regulatory system. DNR1 regulates the expression of DNR2 and DNR2 directly regulates the expression of the denitrification genes in the system. An oxygen-responsive regulator FnrL was indirectly involved in the expression of the denitrification genes via regulation of the biosynthesis of heme, which is required for the activity of DNR. LOV-HK, which is involved in the regulation of the photosynthetic genes, was predicted to be involved in the light-responsive regulation of the denitrification genes in OCh114.
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Free Research Field |
応用微生物学
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