Localization mechanism of yeast membrane proteins to early and late Golgi

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07354
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: NODA YOICHI 東京大学, 大学院農学生命科学研究科(農学部), 助教 (90282699)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 酵母 / 小胞輸送 / ゴルジ体

Outline of Final Research Achievements

To gain insights into the localization mechanisms of membrane proteins to early or late Golgi in yeast, we analyzed the functions and localization of type II Golgi membrane proteins. We tagged the Mnt2 and Mnt3, mannose transferases whose localization mechanism to the Golgi have not been revealed so far, with 3xHA and analyzed the distribution between early and late Golgi cisternae and sought to find the binding proteins to them. Our analysis revealed that Svp26 functioned as ER exit adaptor of Mnt2 and Mnt3. Similar analyses revealed that Svp26 and Mnn6 facilitated the ER exit of Mnn4.

Free Research Field

微生物学