Development of platform technologies to produce fatty acids and biotin by coryneform bacteria

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07356
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shinshu University
• Principal Investigator: IKEDA Masato 信州大学, 学術研究院農学系, 教授 (00377649)
• Co-Investigator(Kenkyū-buntansha): 竹野 誠記 信州大学, 学術研究院農学系, 准教授 (30422702)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: コリネバクテリウム グルタミカム / ビオチン / ピメリン酸 / 脂肪酸

Outline of Final Research Achievements

We engineered naturally biotin-auxotrophic Corynebacterium glutamicum into a biotin prototroph through the heterologous expression of the Escherichia coli bioF gene and the Bacillus subtilis bioI gene. We used the engineered strain to show that carbon flow down the fatty acid-biosynthetic pathway was crucial for biotin biosynthesis. Furthermore, we demonstrated that augmented fatty acid biosynthesis was also reflected on pimelic acid production when carbon flow was blocked at the BioF reaction. These results indicate that the biotin precursor pimeloyl-CoA originates from the fatty acid-biosynthetic pathway in vivo in the engineered biotin-prototrophic C. glutamicum strain.

Free Research Field

応用微生物学