Studies on catabolic pathway of spermidine in E. coli

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: SUZUKI Hideyuki 京都工芸繊維大学, 応用生物学系, 教授 (10202136)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ポリアミン / スペルミジン / アセチルスペルミジン

Outline of Final Research Achievements

The E. coli knockout library was cultured in M9-0.5% glucose medium supplemented with high concentrations of spermidine and 14 strains with decreased acetylspermidine concentration outside the cells were found. However, transporters critical to acetylspermidine efflux could not be found. During this study, a novel transporter SapBCDF which releases putrescine was found.
E. coli deficients in the biosynthetic and catabolic pathways of putrescine was cultured in minimal medium containing high concentration of spermidine but not containing putrescine. Since putrescine was not detected from the culture supernatant nor from the cells, spermidine oxidase was not found under the condition used.

Free Research Field

応用微生物学