Waking up cryptic secondary metabolisms in actinomycetes by a butenolide-type Streptomyces hormone

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07358
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Osaka University
• Principal Investigator: Kitani Shigeru 大阪大学, 生物工学国際交流センター, 准教授 (10379117)
• Co-Investigator(Renkei-kenkyūsha): ARAI Masayoshi 大阪大学, 大学院薬学研究科, 特任教授 (80311231)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 抗生物質生産 / 放線菌 / ブテノライド型シグナル / 潜在二次代謝

Outline of Final Research Achievements

Streptomyces hormones induce the production of secondary metabolites by binding to the cognate receptors in actinomycetes. In this study, I aimed to awaken cryptic useful secondary metabolites through the manipulation of signaling cascades governed by Streptomyces hormones, and to identify actinomycetes strains in which butenolide-type Streptomyces hormones control secondary metabolism. I demonstrated that gene disruption of Streptomyces-hormone receptor activates the production of phthoxazolin A in Streptomyces avermitilis, and that phthoxazolin A biosynthetic genes are encoded on an extra genomic region that is not found in the public database. Furthermore, identification of butenolide-type Streptomyces hormone-like compounds from Streptomyces albus J1074 led to structure-activity relationship for butenolide-type Streptomyces hormones, as well as chemical communication between different streptomycetes via Streptomyces hormones.

Free Research Field

応用微生物学