2017 Fiscal Year Final Research Report
Waking up cryptic secondary metabolisms in actinomycetes by a butenolide-type Streptomyces hormone
Project/Area Number |
15K07358
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Osaka University |
Principal Investigator |
Kitani Shigeru 大阪大学, 生物工学国際交流センター, 准教授 (10379117)
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Co-Investigator(Renkei-kenkyūsha) |
ARAI Masayoshi 大阪大学, 大学院薬学研究科, 特任教授 (80311231)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | 抗生物質生産 / 放線菌 / ブテノライド型シグナル / 潜在二次代謝 |
Outline of Final Research Achievements |
Streptomyces hormones induce the production of secondary metabolites by binding to the cognate receptors in actinomycetes. In this study, I aimed to awaken cryptic useful secondary metabolites through the manipulation of signaling cascades governed by Streptomyces hormones, and to identify actinomycetes strains in which butenolide-type Streptomyces hormones control secondary metabolism. I demonstrated that gene disruption of Streptomyces-hormone receptor activates the production of phthoxazolin A in Streptomyces avermitilis, and that phthoxazolin A biosynthetic genes are encoded on an extra genomic region that is not found in the public database. Furthermore, identification of butenolide-type Streptomyces hormone-like compounds from Streptomyces albus J1074 led to structure-activity relationship for butenolide-type Streptomyces hormones, as well as chemical communication between different streptomycetes via Streptomyces hormones.
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Free Research Field |
応用微生物学
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