Analysis of mesophilic cyanobacterium mutant strain that has acquired a high temperature tolerance comparable to moderately thermophilic bacteria

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07368
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shizuoka University (2017); Tokyo University of Agriculture (2015-2016)
• Principal Investigator: Kanesaki Yu 静岡大学, グリーン科学技術研究所, 特任助教 (70380293)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 適応進化 / シアノバクテリア / ゲノム解析 / 高温ストレス / トランスクリプトーム / 突然変異

Outline of Final Research Achievements

The cyanobacterium Synechococcus elongatus PCC 7942 is the mesophilic photosynthetic organism. We tried long-term adaptive evolution to obtain mutants which are able to proliferate more than a growth limit of high temperature, 43℃. We successfully obtained several mutants which are able to growth more than 45℃ on the BG-11 agar plate. These mutants showed unique transcriptional profile comparing to that of wild-type cells. We identified all spontaneous mutated loci in the genome of the mutant cells by resequence analysis using NGS technology. By verification of the mutations, We found that two genes are associated to the phenotype of cellular thermo-tolerance.

Free Research Field

微生物学