Pioneer research of transcription network of stringent control in Bacillus subtilis and its application

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07374
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokai University (2017); Fukuyama University (2015-2016)
• Principal Investigator: FUJITA Yasutaro 東海大学, 海洋研究所, 客員教授 (40115506)
• Co-Investigator(Kenkyū-buntansha): 広岡 和丈 福山大学, 生命工学部, 准教授 (20389068) ; 小倉 光雄 東海大学, 海洋研究所, 教授 (80204163)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 枯草菌 / 胞子形成 / バイオフィルム / 緊縮応答 / 緊縮転写制御 / 発現制御 / ゲノム / プリン代謝制御

Outline of Final Research Achievements

Transcription initiation of sporulation trigger genes of Bacillus subtilis (kinB and kinA) from adenine (+1) is activated upon stringent response, that is, under positive stringent transcription control. kinB transcription is repressed by a regulatory factor SinR for biofilm formation. Moreover, SinR was found to be involved in positive stringent transcription controlof kinB. The lacZ-fusion and EMSA analyses revealed a SinR-binding site (-61/-57) for SinR repression of kinB and another SinR-binding site (-29/-8) for positive strigent transcription control of kinB. Furthermore, SinR was involved not only in positive stringent transcription control of kinB but also in that of kinA, ilvB, and pycA.

Free Research Field

農学