Analysis of NAD metabolic pathway in Mycobacterium tuberculosis and elucidation of its compensatory pathway

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07377
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MORI Shigetarou 国立感染症研究所, 細菌第二部, 室長 (60425676)
• Project Period (FY): 2015-10-21 – 2018-03-31
• Keywords: 結核菌 / タンパク質 / 酵素 / ドッキングシミュレーション

Outline of Final Research Achievements

In this research project, we mainly analyzed the functions and structures of mycobacterial enzymes involved in the NAD biosynthetic pathway. The optimal conditions for expressing Rv0212c, Rv1901c, Rv3199c, and Rv3393 of Mycobacterium tuberculosis in Escherichia coli were determined. We found that Rv3393 shows nucleoside hydrolysis activity. In addition, we showed that the structure of the substrate-binding site of phosphoribosyl diphosphate synthase from M. smegmatis differs from that of human phosphoribosyl diphosphate synthase. Furthermore, the enzymatic properties of Rv3257c and Rv3308 from M. tuberculosis and the three-dimensional structure of the nucleotide phosphorylase from M. avium were determined in detail.

Free Research Field

構造生物学、細菌学