Biochemical analysis of the generation of high density lipoprotein(HDL) by ABC proteins

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07390
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Kimura Yasuhisa 京都大学, 農学研究科, 助教 (10415143)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ABCタンパク質 / HDL / コレステロール / 脂質輸送 / トランスポーター

Outline of Final Research Achievements

High density lipoprotein (HDL) is the sole pathway to remove the excess cholesterol from peripheral tissues. The amounts/production of HDL reversely correlated to atherosclerosis, HDL is also known as “good cholesterol”. In this study, we performed biochemical analysis of ATP-dependent lipid transporter, ABCA1. We performed the biochemical analysis for ABCA1 and apoA-I interaction. We also demonstrated that ABCA1 temporarily sequesters phospholipids and cholesterol in large extracellular domain and generates HDL by using these lipid. We also purified several lipid transporting ABC transporters and analyzed their substrate specificity.

Free Research Field

生化学