Symbiosis between bifidobacterial species by the analysis of the degradative enzymes for plant glycoprotein

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07397
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kagoshima University
• Principal Investigator: FUJITA Kiyotaka 鹿児島大学, 農水産獣医学域農学系, 准教授 (20381189)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 糖質分解酵素 / プレバイオティクス / アラビノガラクタン-プロテイン / ビフィズス菌 / 腸内細菌 / L-アラビノシダーゼ / 植物糖タンパク質 / L-アラビノース

Outline of Final Research Achievements

L-Arabinose is a widely occurring sugar in plant cell wall polysaccharides and proteoglycans such as arabinan, arabinoxylan, arabinogalactan protein (AGP), and extensin. Bifidobacterium adolescentis encodes a gene cluster for L-arabinose degradative enzymes. In this study, we cloned and characterized GH27 β-L-arabinopyranosidase (BAD_1525), GH51 α-L-arabinofuranosidase (BAD_1524), GH43 α-L-arabinofuranosidase (BAD_1527), GH36 β-L-arabinopyranosidase (BAD_1528), and GH127β-L-arabinofuranosidase (BAD_1529) from B. adolescentis JCM1275.

Free Research Field

糖質科学