Molecular functional analysis of alminum inhibition and transport of plant magnesium channel MRS2

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07399
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Ishijima Sumio 京都府立大学, 生命環境科学研究科, 准教授 (70184520)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: マグネシウムイオン / 膜輸送タンパク質 / アルミニウム / シロイヌナズナ / イネ / リポソーム

Outline of Final Research Achievements

Plant MRS2 proteins may play a role in Mg2+ transport. Magnesium and aluminum transport activity and Al inhibition of Mg2+ transport of 3 Arabidopsis thaliana MRS2 proteins and 2 Oryza sativa MRS2 proteins were analyzed using purified MRS2 proteins reconstituted into liposomes and functional complementation assay with Escherichia coli mutants. The results showed that these MRS2 proteins could transport Mg2+, but their Al sensitivity was much different among the MRS2 proteins.

Free Research Field

生化学、分子生物学