2017 Fiscal Year Final Research Report
Molecular evolution of auxin biosynthetic enzymes in insects, and their causal relations to acquirement of gall inducing ability by galling insects
| Project/Area Number |
15K07405
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Research Collaborator |
ARAI Ryoma
YOKOYAMA Chiaki
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ゴール / オーキシン / 生合成 / Bombyx mori / Pontania sp. |
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| Outline of Final Research Achievements |
We previously showed that galling insects biosynthesize auxin, a class of phytohormones, and accumulate it at high concentrations. We have also proposed the highly probable auxin biosynthetic pathway in insects, and have succeeded in obtaining a biosynthetic inhibitor. In this study, we tried to demonstrate the importance of auxin for gall induction by using the inhibitor, but it was clarified that some substances to cancel the function of the inhibitor exist in hemolymph, resulting in the failure in inducing auxin defect in insects in vivo. On the other hand, we have successfully interpreted the reason why biosynthetic intermediates cannot be detected by showing that their metabolizing rates were much faster than their producing rates. We have also successfully identified an enzyme involved in the conversion of indole-3-acetaldehyde to indole-3-acetic acid, the active auxin, from Bombyx mori silk-gland extract.
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| Free Research Field |
農学
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