Study of acrylamide metabolism genes and research on effective breeding method in practical Aspergillus oryzae

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07463
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kanazawa Institute of Technology
• Principal Investigator: Kenji Ozeki 金沢工業大学, バイオ・化学部, 教授 (40410287)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 麹菌 / アクリルアミド / アミダーゼ / アクリル酸

Outline of Final Research Achievements

ASC5-4 which is a high producing strain of acrylic acid (AS) degradation candidate in Aspergillus oryzae (Self Cloning) capable of degrading acrylamide (AA) can increase the efficiency of AS reduction in water and coffee I found out. There was no particular phenomenon in which efficiency was improved for reducing AA. It was found that the Aspergillus oryzae bred by this was one of the genes related to AS degradation. However, they work separately without interlocking with amidase, and it seems that there is a problem with localization of each enzyme in the cell. Establishment of conditions for repeated reuse of amidase high production Aspergillus oryzae and prospect of technological development to suppress the disposal treatment of cellulose carrier.

Free Research Field

応用微生物