Cell biological studies on behavior of autolytic enzymes that control heartwood formation in trees

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07508
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Satoshi Nakaba 東京農工大学, (連合)農学研究科(研究院), 准教授 (40627709)
• Co-Investigator(Renkei-kenkyūsha): TAKATA Naoki 森林総合研究所, 森林バイオ研究センター, 主任研究員 (90605544)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 心材形成 / 放射柔細胞 / 細胞死 / 自己分解酵素 / ショットガンプロテオーム解析 / 遺伝子発現解析 / 形質転換体 / ポプラ

Outline of Final Research Achievements

In the present study, we showed radial variation of the content of proteins in ray parenchyma cells in sapwood of Populus suaveolens by shotgun proteomic analysis. In addition, we found that contents of several proteases increase towards inner part of sapwood. This result suggests that these proteases might be involved in autolysis in the process of cell death in ray parenchyma cells. Then, we showed radial variation of gene expressions of these proteases in ray parenchyma cells by real-time PCR analysis. Moreover, we made transgenic plants of P. tremula x P. tremuloides to understand expression and localization of xylem cysteine peptidase (XCP), which is a kind of important autolytic enzyme in programmed cell death of short-lived tracheary element. Then, we showed localization of the expression of genes of XCP in ray parenchyma cells in these transgenic plants.

Free Research Field

木質科学