2017 Fiscal Year Final Research Report
Development of Rhizogon roseolus strains showing salt tolerance and vigorous mycelial growth characteristics in order to reconstruct coastal and forest regions
| Project/Area Number |
15K07512
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Keywords | ショウロ / 外生菌根菌 / 担子胞子発芽 / 突然変異 / 塩ストレス耐性 / ナトリウム取込み |
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| Outline of Final Research Achievements |
Basidiospore germination rate of Rhizopogone roseolus was improved by using 1/5-diluted MMN agar media and fruiting bodies with beige-colored section. Salt-tolerant homokaryotic strains were isolated from the basidiospore suspension treated with ethylmethanesulfonate. Salt-tolerant heterokaryotic strains were obtained by crossing between the salt-tolerant homokaryotic strains. We have selected the strains showing two useful characteristics, i.e. sat tolerance and vigorous mycelial growth. Pinus thunbergii seedlings inoculated with the salt-tolerant strain of R. roseolus were tolerant against salt stress, unlike the P. thunbergii seedlings inoculated with the salt-sensitive one. The sodium content in P. thunbergii seedlings inoculated with R. roseolus was greater than that in P. thunbergii seedlings inoculated without R. roseolus, indicating that the ectomycorrhizas formed by R. roseolus did not inhibit sodium uptake in the host P. thunbergii seedlings.
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| Free Research Field |
きのこ栽培学
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