2017 Fiscal Year Final Research Report
Why can't mushrooms ferment ethanol ?
| Project/Area Number |
15K07514
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | きのこ / エタノール / アルコール脱水素酵素 / ピルビン酸脱炭酸酵素 / アルデヒド脱水素酵素 / グリセルアルデヒドリン酸脱水素酵素 / ナメコ / 発現解析 |
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| Outline of Final Research Achievements |
In order to answer the question "Why mushrooms cannot ferment ethanol?", i)pyruvate decarboxylase, ii) alcohol dehydrogenase, and iii) aldehyde dehydrogenase genes which are key enzymes of alcohol production, were identified and their expression were analyzed.As the result, the expression of pyruvate decarboxylase gene is very weak, and it was strongly suggested that the role of alcohol dehydrogenase and aldehyde dehydrogenase has evolved from ethanol metabolism to decomposition of aromatic compounds.
Therefore, expression of alcohol dehydrogenase gene and pyruvate decarboxylase gene isolated from baker’s yeast under the control of glyceraldehyde-3-phosphate dehydrogenase promoter from Nameko, are to be investigated to breed ethanol fermentable mushroom
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| Free Research Field |
菌類分子遺伝学
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