A study of analysis and utilization of ligninolysis induced by over-expression of a catalytic subunit of protein kinase A.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07517
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: The University of Shiga Prefecture
• Principal Investigator: IRIE TOSHIKAZU 滋賀県立大学, 環境科学部, 准教授 (30336721)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: リグニン / きのこ / 白色腐朽菌 / バイオマス変換

Outline of Final Research Achievements

Results of this study collectively suggest that protein kinase A has an import role for expression of ligninolysis in white-rot fungi. Novel selectable maker gene, nourseothricin resistant gene was successfully used for transformation of Pleurotus ostreatus. In addition, the efficiency of P. ostreatus transformation using bialaphos-resistant gene as a selectable maker was improved by our new method. With the aim of construction of custom recombinant strain of P. ostreatus used for conversion of weeds to fish feeds, nitrogen-catabolite repression system of the fungus was analyzed. As the result, it is suggested that P. ostreatus also has a nitrogen-catabolite repression system regulated by AreA (nit2) like as that in ascomycete.

Free Research Field

応用微生物学