Whole genome determination and vaccination of kuchijirosho virus.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07558
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Fukui Prefectural University
• Principal Investigator: Miyadai Toshiaki 福井県立大学, 海洋生物資源学部, 名誉教授 (20157663)
• Co-Investigator(Kenkyū-buntansha): 末武 弘章 福井県立大学, 海洋生物資源学部, 准教授 (00334326)
• Co-Investigator(Renkei-kenkyūsha): ISSHIKI Tadashi 三重大学, 大学院生物資源学研究科, 准教授 (30378319) ; YOSHIDA Takashi 京都大学, 大学院農学研究科, 准教授 (80305490)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 口白症 / トラフグ / ウイルス / ゲノム / ワクチン

Outline of Final Research Achievements

Three types of RNA (kuchijirosho associated RNA: KAR) appeared in the kuchijirosho-affected fugu brain. BLAST analysis of nucleotide and amino acid sequence showed that KARs are not similar to any other viruses registrated in the database, suggesting that kuchijirosho virus may be cllasified into a new category of virus. Swiss-Plot analysis indicated that KAR coding proteins possess viral RNA polymerase or arginine kinase; however, we could not find RNA encoding capsid protein or envelope protein.
Formalin treated cell culture infected with kuchijirosho virus had no effect as vaccine.

Free Research Field

水族病理学