• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2017 Fiscal Year Final Research Report

The role of relaxin-like factor (RLF)/insulin-like factor 3 (INSL3) as germ cell anti-apoptotic and maturation factor on boar sperm production

Research Project

  • PDF
Project/Area Number 15K07691
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal production science
Research InstitutionShizuoka University

Principal Investigator

Kohsaka Tetsuya  静岡大学, 農学部, 教授 (10186611)

Research Collaborator SASADA Hiroshi  
SHIBATA Masatoshi  
TERADA Kei  
Project Period (FY) 2015-04-01 – 2018-03-31
Keywordsリラキシン / RLF / INSL3 / Passive immunization / Active immunization / Sperm production / Germ cell apoptosis
Outline of Final Research Achievements

This study was to produce model pigs neutralized endogenous RLF by immunization with RLF/INSL3 antibody and to investigate the mechanism of its action as germ cell anti-apoptotic and maturation factor. First, RLF inactivation resulted in a 4-fould increase in the frequency of apoptotic germ cells. Second, inactivation led to upregulation of the pro-apoptotic BAX and downregulation of the anti-apoptotic BCL2, and in turn activated the mitochondrial apoptotic pathway with induction of CASP3. Third, neutralization affected the development of epididymal muscle layer. Finally, inactivation had no effect on semen volume; however, it caused a significant reduction in sperm motility, concentration and quality compared with control. These results revealed that the RLF/INSL3 inactivation (neutralization) increases germ cell apoptosis and decreases in semen quality and sperm output, suggesting that RLF/INSL3 acts as an germ cell anti-apoptotic and maturation factor of sperm production.

Free Research Field

動物生殖生理学

URL: 

Published: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi