2016 Fiscal Year Research-status Report
Mechanisms and functions of neuropeptides and amino acids to adapt heat-stress
| Project/Area Number |
15K07694
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
豊後 貴嗣 広島大学, 生物圏科学研究科, 教授 (40325361)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Heat-stress / Chicks / Broilers / Thermotolerance / NPY / L-Leucine / L-Citrulline / Metabolism |
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| Outline of Annual Research Achievements |
Last year, in 2016, I reported that we found the anti-stress functions of neuroppetide Y(NP)Y in heat-exposed chicks. We have published an international paper on this findings (Bahry et al., in press, Neuropeptides). I am also continuing works on NPY and recently my student gave a talk in Japan Poultry Science Association at Kobe with the new findings of NPY in heat stress chicks. Interestingly, it was found that NPY provided thermotolerance in heat exposed chicks. NPY central injection also caused hypoglycemia, which would be a reason of hypothermia because previously it was reported in many publications that hypoglycemia and hypothermia has strong relationship. I am planning to measure plasma insulin in NPY injected chicks to understand the reason of hypoglycemia. NPY central injection and heat stress caused to stimulate three NPY receptors (NPY-R5, R6 and R7), but not others. I am now progressing to buy first the NPY-R5 antagonist to check its involvement in the NPY-dependent thermoregulation process since NPY-R6 and R7 antagonist are still not available. On the other hand, gonadotropin-inhibitory hormone (GnIH) paper is under preparation. As for amino acids, L-leucine (L-Leu) has been found as a strong agent to afford thermotolerance (Han et al., 2017; Comp. Biochem. Physiol. A). L-Citrulline (L-Cit) also afforded thermotolerance even in 4 weeks old broilers. L-Cit study has been conducted with my collaborator (Buntansha) Dr. T. Bungo.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Currently, an Egyptian student is actively working on NPY research. L-Leucine in ovo administration study with thermotolerance is carrying out by a Chinese doctoral student. L-Citrulline (L-Cit) study focusing with the natural source of L-Cit is being carrying out by a Vietnamese doctoral student. I am conducting research with synthetic L-Cit in collaboration with Dr. Bungo (Buntansha) using broilers at Hiroshima Univ. One of my Master students has started works with heat stress in goldfish. He has found interesting results on oxygen consumption was increased under heat stress.
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| Strategy for Future Research Activity |
NPY receptor antagonist works is under progress, which will clarify the mechanism of NPY-dependent hypothermia and thermotolerance. L-Leucine (L-Leu) and L-citrulline (L-Cit) related to thermotolerance mechanisms are also under progress. Gonadotropin-inhibitiry hormone (GnIH) paper and L-Cit findings are under preparation of manuscript. In this year, I will try my best to conclude the mechanisms of NPY, L-Leu and L-Cit in thermoregulation and thermotolerance process.
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| Causes of Carryover |
Since, reagents (NPY, antagonist, PCR, amino aicds, HPLC, etc.), chicks, broilers are costly items, I need budget to carry out my research works. I also need to travel to Hiroshima Univ. for collaborative works on broilers. Funds also needed to publish papers, reprints and conference presentations.
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| Expenditure Plan for Carryover Budget |
I am planning to work with NPY R5, plasma insulin, and HPLC analysis of free amino acids in NPY injected chicks. I am also planning to analyze microarray to identify the genes regulated by L-leucine in ovo injection under heat stress condition. I have another plan on L-citrulline administration to develop thermotolerance.
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