The research for relationships among genome DNA methylation polymorphism and marbling in Japanese beef cattle

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07696
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Miyagi University
• Principal Investigator: Yoshihito Suda 宮城大学, 食産業学群(部), 教授 (90404847)
• Co-Investigator(Kenkyū-buntansha): 小林 栄治 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, ユニット長 (00186727)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 黒毛和種 / DNA / メチル化 / 多型 / 脂肪交雑 / 枝肉成績

Outline of Final Research Achievements

This study’s aims are to examine relationships among DNA methylation patterns at the slaughter after fattening and economic carcass traits of JB siblings of two sires. Genomic DNA samples were collected from adipose tissues around the kidneys in each 100 JBs produced from 2 sires (Sire A and B) have parent-child relation, totaling of 200 JBs. Correlation analysis and analysis of variance (ANOVA) are performed by using SAS program, version 9.1. The CG rich regions in the upstream regions of seven genes, APC_2, CDKN2A_1, CDKN2A_2, CDKN2B, GSTP1, MGMT and SFRP1 of 24 genes were very high DNA methylation level, and DMP in the other genes was similar between 2 sires with parent-child relation. Additionally, CDKN2A_2 and RB1 was specific condition. Correlation between subcutaneous fat thickness and CHFR was 0.73 at 5% significance level in JBs of sire A.

Free Research Field

動物遺伝育種学