2017 Fiscal Year Final Research Report
Studies on phenotypes of Listeria monocytogens grown under room temperature and refrigeration conditions
| Project/Area Number |
15K07729
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | リステリア / 酸 / 過酸化水素 / 温度 / ストレス |
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| Outline of Final Research Achievements |
Listeria monocytogenes is a food-borne pathogen. In the present study, L. monocytogenes grown at 20℃ or 37℃ was subjected to low pH and hydrogen peroxide under different temperatures, including 20℃ and 37℃. Survival curves indicated an increase in the resistance of L. monocytogenes to hydrogen peroxide as the temperature decreased during the procedure of stress exposure. In addition, transcriptional induction of some genes that have central roles in response to hydrogen peroxide exposure, including kat and sigB, was observed at 37℃ but not at 20℃, whereas other stress response genes that were involved in gene expression during the stress exposure were induced at the both temperatures. These findings suggest that hydrogen peroxide is not recognized as a potent stress factor under room temperature conditions. Therefore, this study suggests that this pathogen exerts the high ability to survive and propagate in food processing environments.
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| Free Research Field |
獣医公衆衛生学
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