Investigation of erythroid differentiation using transgene-free canine and feline induced pluripotent stem cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07747
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: Hatoya Shingo 大阪府立大学, 生命環境科学研究科, 准教授 (40453138)
• Co-Investigator(Kenkyū-buntansha): 杉浦 喜久弥 大阪府立大学, 生命環境科学研究科, 教授 (30171143) ; 竹中 重雄 大阪府立大学, 総合リハビリテーション学研究科, 教授 (10280067)
• Co-Investigator(Renkei-kenkyūsha): NAKANISHI Mahito 国立研究開発法人産業技術総合研究所, その他部局等, 研究員 (10172355) ; HISASUE Masaharu 麻布大学, 獣医学部, 准教授 (80333144)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: iPS細胞 / 多能性幹細胞 / 再生医療 / 獣医学 / イヌ / ネコ / 血液

Outline of Final Research Achievements

The objective of this study was to establish genome integration-free canine and feline induced pluripotent stem cells (ciPSCs), and to induce hematopoietic cells from these stem cells. The following results were obtained.
①We confirmed that the Sendai virus vector (SeVdp) could infect canine and feline embryonic fibroblasts by detecting GFP expression. ②We could reprogram feline embryonic fibroblasts into iPS-like cells using SeVdp containing 6 transcription factors with TGF-beta inhibitor. These cells were positive for pluripotent markers. ③We transduced four transcriptional factors into canine fibroblast cells with SeVdp, and could established iPS-like cell line using TGF-beta inhibitor. Finally, we reprogrammed canine embryonic fibroblasts using the auto-erasable defective and persistent SeVdp, and canine iPS-like cells were formed. Further studies are required to induce hematopoietic cells from these canine or feline stem cells.

Free Research Field

獣医学