Establishment of regenerative medicine for spinal cord injury using bFGF without stem cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07752
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Nihon University
• Principal Investigator: EDAMURA Kazuya 日本大学, 生物資源科学部, 准教授 (80366624)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 犬 / 塩基性線維芽細胞増殖因子 / 再生医療 / 骨髄間質細胞 / 脊髄損傷 / 神経再生 / 動物 / 無細胞蛋白質合成技術

Outline of Final Research Achievements

Canine bone marrow stromal cells (BMSCs) were incubated with the basal medium for neurons containing recombinant human basic fibroblast growth factor (rh-bFGF). The rh-bFGF treatment resulted in the differentiation of canine BMSCs into voltage- and glutamate-responsive neuron-like cells, and we also clarified a pat of the mechanism. In this study, canine recombinant basic fibroblast growth factor (rc-bFGF) was synthesized with a wheat germ cell-free protein synthesis system. When our synthesized protein was added to HEK293 cells, the expression of pERK was confirmed. The result suggested that our synthesized protein was rc-bFGF and had the functions as bFGF. When canine BMSCs were incubated with bFGF, the cell shape changed to neuron-like morphology. In addition, rc-bFGF induced differentiation of canine BMSCs into voltage- and glutamate-responsive neurons. The rc-bFGF might contribute to regenerative therapy for neuron in dogs.

Free Research Field

獣医外科学