Roles of membrane rafts in molecular basis of fertilition in avian sperm

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07761
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: University of Tsukuba
• Principal Investigator: ASANO Atsushi 筑波大学, 生命環境系, 助教 (10630981)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 精子 / 先体反応 / 鳥類 / 細胞内シグナリンク / 細胞膜マイクロドメイン / ステロール

Outline of Final Research Achievements

①Qualitative analysis of functional roles of membrane rafts was conducted using quantitative proteomics combined with dimethyl-labeling approach, demonstrating at least 82 proteins are relatively or exclusively enriched in membrane rafts.
②Membrane rafts regulate adenyl cyclase isoforms, such as sAC and tmAC, and glucose transporter activities, thereby stimulating acrosome reaction induction in chicken sperm.
③ Above results led to development of avian sperm cryopreservation and demonstrated that adding sterols into the plasma membranes of chicken sperm dramatically improves the post-thaw semen quality.

Free Research Field

生殖生物学